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KMID : 0391319920020020183
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Korean Journal of Biological Response Modifiers
1992 Volume.2 No. 2 p.183 ~ p.192
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cis Urocanic Acid Induced Immunosuppression is Associated with A Prostaglandin-Dependent Mechanism
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전병득
김종수/박천수/강경진/이무삼
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Abstract
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trans-Urocanic acid (UCA) is isomerized to cis-UCA by ultraviolet radiation (UVR) It was suggested that UVR conversion cis-UCA function as a "photorecepter" to induce immune suppression Some investigators have shown that prostaglandins (PG) are
involved
in UVR-immune suppression of contact hypersensitivity (CH) The purpose of this study was to determine whether cis-UCA caused suppression of CH by a PG dependent mechanism trans UCA (4 mg/ml 10% DMSO in saline) was exposed to 5.4kl/㎡
UVR(280.320nm),
which induced 60% cis-UCA conversion Mice were injected subcutaneously with 25μl 0.5% DNFB. After 5 days they were challenged on the right ear and ear swelling responses (units of 10E-4 inches)were compared between UCA treated and untreated
controls,
24 hrs after challenge Control mice had 90 U of ear swelling compared to 78 U for trans-UCA (15% suppresion) and 49 U for cis-UCA (49% suppression) treated mice. To determine whether PG were involved, 1 day before UCAtreatments mice received a
subcutaneous implant of a indomethacin containing pellet (2.5 ㎍ indomethacin released per day). Compared to controls it was found that indomethacin had prevented cis-ICA induced immune suppression, i. e. CH of trans-UCA mice was 73 U and cis-UCA
mice
was 74 U a level of suppression of 19%. These results support the hypothesis that UVR-induced cis-UCA is capable of causing suppression of CH, however, it mediates its suppressive effect though a PG dependent mechanism.
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KEYWORD
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